Wednesday, 4 July 2018

Kefir Supplementation Modifies Gut Microbiota Composition, Reduces Physical Fatigue, and Improves Exercise Performance in Mice

one more interesting paper on the value of fermented dairy products

Kefir Supplementation Modifies Gut Microbiota Composition, Reduces Physical Fatigue, and Improves Exercise Performance in Mice

(a) Effects of kefir (KF) supplementation on principal coordinate analysis (PCoA) of the gut microbiota composition in mice based on the Bray–Curtis distance measure of samples in the relative abundance profiles of an operational taxonomic unit; (b) Effects of KF supplementation on the phylum-level gut microbiota composition of mice. Only phyla with top 10 average abundances were included, with other phyla collapsed into “Others”; the effects of KF supplementation on the phylum-level gut microbiota composition of mice in the (c) KF-1X and vehicle groups; (d) KF-2X and vehicle groups, and (e) KF-5X and vehicle groups. The cladogram was generated from the linear discriminant analysis effect size (LEfSe) analysis, showing the most differentially abundant taxa enriched in the microbiota of mice in (c) the KF-1X (red) and vehicle (green) groups, (d) the KF-2X (red) and vehicle (green) groups, and (e) the KF-5X (red) and vehicle (green) groups. Significant differential abundances in LEfSe comparisons are sorted by p values in ascending order: vehicle (glucose water), KF-1X (2.15 g/kg/day KF), KF-2X (4.31 g/kg/day KF), and KF-5X (10.76 g/kg/day KF).


 
Abstract 
 
The present study evaluated the potential beneficial effect of kefir (KF) against fatigue. Furthermore, the composition of the gut microbiota is related to health benefits in the host; therefore, the study also investigated the effect of KF on the gut microbiota composition. Male ICR mice from four groups (n = 8 per group) were orally administered KF once daily for four weeks at 0, 2.15, 4.31, and 10.76 g/kg/day and were designated as the vehicle, KF-1X, KF-2X, and KF-5X groups, respectively. The gut microbiota was analyzed using 16S rRNA gene sequencing. The results showed a significant clustering of cecum after treatment in the vehicle, KF-1X, KF-2X, and KF-5X groups. The KF-2X and KF-5X groups showed a decreased Firmicutes/Bacteroidetes ratio compared with the vehicle group. In addition, anti-fatigue activity and exercise performance were evaluated on the basis of exhaustive swimming time, forelimb grip strength, and levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a swimming exercise. The exhaustive swimming time for the KF-1X, KF-2X, and KF-5X groups was significantly longer than that for the vehicle group, and the forelimb grip strength of the KF-1X, KF-2X, and KF-5X groups was also significantly higher than that of the vehicle group. KF supplementation also decreased serum lactate, ammonia, BUN, and CK levels after the swimming test. However, tissue glycogen content, an important energy source for exercise, increased significantly with KF supplementation. Thus, KF supplementation can alter the gut microbiota composition, improve performance, and combat physical fatigue. View Full-Text
Post a Comment