This is the question we have tried to answer in this paper that has just been published in
Food Research International.
Structure and cardioprotective
activities of polar lipids of olive pomace, olive pomace-enriched fish
feed and olive pomace fed gilthead sea bream (Sparus aurata)
Highlights
- •
- Gilthead sea bream was aquacultured using 8% olive pomace in fish finishing diet.
- •
- Polar lipids of all samples were assessed in vitro towards platelet aggregation.
- •
- These polar lipid fractions were also studied by ES-MS/MS and GC–MS.
- •
- Major classes of diacyl-glycerophospholipids (PC, PE, PI, PG, PA) were identified.
- •
- Two PE species were identified in OP, OP-diet and fish fed with OP-diet.
Abstract
Total
lipids of olive pomace (OP), olive pomace diet (OP diet), fish oil diet
(FO diet) and fish filets of farmed gilthead sea bream (fish fed with
FO diet and OP diet respectively) were extracted and separated into
polar (TPL) and neutral (TNL) lipids. All samples were assessed for
their in vitro activity against washed rabbit platelets aggregation
induced by platelet activating factor (PAF) and they were further
analyzed by electrospray-mass spectrometry. The high levels of palmitic
(16:0), oleic (18:1 cis ω − 9), linoleic (18:2 ω − 6) and
docosapentaenoic acid (DPA 22:5 ω − 3) contained in both OP and FO diets
are reflected to the gilthead sea breams fed with the individual diet
respectively, while the gilthead sea bream fed with FO diet displays a
decrease in DPA. All samples contained various glycerophospholipids
species. Two PE species were identified in OP, OP diet and fish fed with
OP diet and not in FO diet, while that might be an indication that
these substances are likely to be the key polar phospholipids that have
the ability to be in vitro PAF inhibitors, i.e. inhibit the formation of
atherosclerotic plaques in blood arteries.
= = =
The related patent can be found here.
