New paper of our team

In Vitro Antithrombotic Properties of Salmon (Salmo salar) Phospholipids in a Novel Food-Grade Extract

 

Alexandros Tsoupras ^1,†, Ronan Lordan ^1,†, Katie Shiels ², Sushanta Kumar Saha ², Constantina Nasopoulou ^3,* and Ioannis Zabetakis ¹

Abstract

Marine and salmon polar lipids (PLs) extracted by conventional extractions with non-food-grade solvents (CE-salmon-PLs) possess antithrombotic bioactivities against platelet-activating factor (PAF) and thrombin. Similar effects of food-grade-extracted (FGE) marine PLs have not yet been reported. 

In this study, food-grade solvents were used to extract PLs from Irish organic farmed salmon (Salmo salar) fillets (FGE-salmon-PLs), while their antithrombotic bioactivities were assessed in human platelets induced by platelet aggregation agonists (PAF/thrombin). FGE-salmon-PLs were further separated by thin layer chromatography (TLC) into lipid subclasses, and the antithrombotic bioactivities of each subclass were also assessed. 

LC-MS was utilized to elucidate the structure-activity relationships. FGE-salmon-PLs strongly inhibited PAF-induced platelet aggregation, while their relevant anti-thrombin effects were at least three times more potent than the previously reported activities of CE-salmon-PLs. TLC-derived lipid fractions corresponding to phosphatidylcholines (PC) and phosphatidylethanolamines (PE) were the most bioactive lipid subclasses obtained, especially against thrombin. Their LC-MS analysis elucidated that they are diacyl- or alkyl-acyl- PC and PE moieties baring ω3 polyunsaturated fatty acids (PUFA) at their sn-2 position, such as eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA). 

Our results concerning the potent antithrombotic effects of FGE-salmon-PLs against both PAF and thrombin pathways strongly suggest that such food-grade extracts are putative candidates for the development of novel cardioprotective supplements and nutraceuticals. 

Keywords: salmon; polar lipids; platelet aggregation; platelet-activating factor (PAF); thrombin; phosphatidylcholine; phosphatidylethanolamine; LC-MS; EPA; DHA; PUFA; phospholipids 

how can we produce sustainably nutraceuticals?

 

 

Omega-3 withdrawal by retailers opens up sustainability dialogue

16-Apr-2018 By Will Chu

UK health and beauty retailer Boots have become the latest target of a campaign by Greenpeace to halt the sale of Omega-3 supplements derived from krill oil fished in vulnerable marine areas. 

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this news ...bring back the question:  

how can we produce sustainably nutraceuticals?

[watch this space ; in few months we are going to have the complete answer]