Caprine milk fermentation enhances the antithrombotic properties of cheese polar lipids
Abstract
The effect of fermentation on the
antithrombotic properties of polar lipids in raw and pasteurised caprine
milk was assessed through the production of various cheeses using the
same starter culture. The total lipids (TL), total neutral lipids (TNL),
and total polar lipids (TPL) were extracted from each milk and cheese
and the TPL fatty acid profiles were analysed by GC–MS. It was
determined that fermentation influenced the polar lipid fatty acid
composition. The milk and cheese polar lipids exhibited potent
antithrombotic activities with IC50 values ranging from 79 to
226 µg against platelet-activating factor (PAF) induced platelet
aggregation. Finally, shotgun metagenomics determined the species-level
microbial composition and functional potential of each milk and cheese.
Several microbe-encoded phospholipid biosynthetic genes were identified
in the most antithrombotic cheeses. Lactococcus lactis and other
microbial species may play a significant role in determining the
antithrombotic properties and fatty acid composition of caprine cheese
polar lipids.
© 2019 Elsevier Ltd
