just published in Marine Drugs
Department of Biological Sciences, University of Limerick, V94 T9PX Limerick, Ireland
Department of Life and Environmental Sciences, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy
Shannon Applied Biotechnology Centre, Limerick Institute of Technology, Moylish Park, V94 E8YF Limerick, Ireland
Department of Food Science and Nutrition, School of the Environment, University of the Aegean, GR 81400 Myrina, Lemnos, Greece
Author to whom correspondence should be addressed.
Received: 17 April 2018 / Revised: 16 May 2018 / Accepted: 22 May 2018 / Published: 23 May 2018
(This article belongs to the Special Issue Marine-derived Bioactive Peptides and Lipids Applied in Non-communicable Diseases)
AbstractWhile several marine polar lipids (PL) have exhibited cardioprotective properties through their effects on the platelet-activating factor (PAF) pathways, salmon PL have not been tested so far. In this study, the antithrombotic activities of salmon PL were assessed in human platelets and the structural characterisation of bioactive salmon PL was performed by GC-MS and LC-MS analyses. PL from fillets of Irish organic farmed salmon (Salmo salar) were extracted and separated into several lipid subclasses by thin-layer chromatography (TLC), while their fatty acid profile was fully characterised by GC-MS. Salmon total lipids (TL), total neutral lipids (TNL), total polar lipids (TPL), and each PL subclass obtained by TLC were further assessed for their in vitro effects towards PAF-induced and thrombin-induced platelet aggregation in human platelets. Salmon PL exhibited antithrombotic effects on human platelet aggregation, mostly through their strong inhibitory effects against the PAF pathway with IC50 values comparable to other marine PL, but with lower effects towards the thrombin pathway. PL fractions corresponding to phosphatidylcholine and phosphatidylethanolamine derivatives exhibited the most potent anti-PAF effects, while LC-MS analysis putatively elucidated their structure/function relationship. Several diacyl-PC/PE and alkyl-acyl-PC/PE species containing mostly docosahexaenoic acid at their sn-2 glycerol-backbone may be responsible for the bioactivity. The data presented suggests that salmon contains PL with strong antithrombotic bioactivities.
Keywords: salmon; polar lipids; platelet aggregation; platelet-activating factor (PAF); thrombin; antithrombotic; LC-MS; GC-MS; phosphatidylcholine; phosphatidylethanolamine; polyunsaturated fatty acids (PUFA); docosahexaenoic acid (DHA)